Strategies for the NMR‐Based Identification and Optimization of Allosteric Protein Kinase Inhibitors

Caught coming in the back door. Kinases are important drug targets, and new methods for the identification of novel classes of kinase inhibitors are urgently needed. By using a spin-labeled analogue of adenine (shown as a red star), second-site NMR screening can identify allosteric kinase inhibitors (right) that bind outside of the ATP-binding pocket and might have an interesting selectivity profile.