In vivo 3′-to-5′ exoribonuclease targetomes of Streptococcus pyogenes

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Significance To cope with harsh environments and cause infection, bacteria need to constantly adjust gene expression. Ribonucleases (RNases) control the abundance of regulatory and protein-coding RNA through degradation and maturation. The current characterization of 3′-to-5′ exoribonucleases (exoRNases), processing RNAs from their 3′ end, is solely based on the description of a limited number of targets processed by these RNases. Here, we characterized bacterial 3′-to-5′ exoRNase targetomes. We show that YhaM, polynucleotide phosphorylase (PNPase), and RNase R have exoribonucleolytic activities in the human pathogen Streptococcus pyogenes . We demonstrate that PNPase is the main 3′-to-5′ exoRNase participating in RNA decay, we show that RNase R has a limited processing activity, and we describe an intriguing RNA processing behavior for YhaM.