The Effect of the Specific Phosphodiesterase-Iv-Inhibitor Rolipram on the Uretheral Peristalsis of the Rabbit in Vitro and in Vivo

No AccessJournal of UrologyInvestigative Urology1 Sep 1998THE EFFECT OF THE SPECIFIC PHOSPHODIESTERASE-IV-INHIBITOR ROLIPRAM ON THE URETHERAL PERISTALSIS OF THE RABBIT IN VITRO AND IN VIVO ARMIN J. BECKER, CHRISTIAN G. STIEF, MARKUS MEYER, MICHAEL C. TRUSS, WOLF G. FORSSMANN, and UDO JONAS ARMIN J. BECKERARMIN J. BECKER , CHRISTIAN G. STIEFCHRISTIAN G. STIEF , MARKUS MEYERMARKUS MEYER , MICHAEL C. TRUSSMICHAEL C. TRUSS , WOLF G. FORSSMANNWOLF G. FORSSMANN , and UDO JONASUDO JONAS View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62833-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Cyclic nucleotide phosphodiesterases (PDE's) are intracellular key enzymes in the regulation of the tone in smooth muscle cells. There are 5 different isoenzyme families that show a specific organ and species distribution pattern. The aim of our study was to examine possible selective ureteral relaxation by the specific PDE-IV-inhibitor rolipram in the rabbit. Materials and Methods: For in vitro studies ureters were taken from 12 patients who underwent radical nephrectomy and from 4 rabbits which were sacrificed. For the in vivo study 23 rabbits received intravenous administration of papaverine (300 micro g./kg. b.w., n = 2), scopolamine (667 micro g./kg. b.w., n = 2), theophylline (5 mg./kg. b.w., n = 2) and rolipram (5 and 20 micro g./kg. b.w., n = 9 and n = 8). Ureteral dynamic parameters (frequency, amplitude, tonus) and systemic blood pressure were continuously monitored 40 minutes before and at least 60 minutes after injection of the drugs. Results: Scopolamine had no effect on ureteral peristalsis, but significant effects on systemic blood pressure were observed. Papaverine and theophylline showed short-lasting ureteral relaxations, but were accompanied by severe circulatory side effects. Rolipram showed pronounced ureteral relaxation with minimal circulatory effects. Conclusion: Our results show that intravenous administration of the specific PDE-IV-inhibitor rolipram relaxes the rabbit ureter without significant circulatory side effects. Because human and rabbit ureter show similar relaxation results in vitro, this pharmacological approach seems promising for the treatment of ureteral colic and the facilitation of urinary stone passage in humans. References 1 : Management of the stone former. Semin. Urol.1984; 2: 34. Google Scholar 2 : Calcium Urolithiasis. New York: Plenum Press1978. Google Scholar 3 : Urinary lithiasis. In: Campbell's Urology. Edited by . Philadelphia: Saunders Company1986: 1094. Google Scholar 4 : Spasmolysis by beta-adrenergic agents. In: Urodynamics.. Edited by . Heidelberg: Springer Verlag1973. Google Scholar 5 : Phosphodiesterase activity in human ureter and the effect of various selective inhibitors. J. Urol.1993; 149: 274A. 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Google Scholar From the Department of Urology, Medizinische Hochschule, and the Lower Saxony Institute for Peptide Research, Hannover, Germany© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byÜCKERT S, KÜTHE A, JONAS U and STIEF C (2018) CHARACTERIZATION AND FUNCTIONAL RELEVANCE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE ISOENZYMES OF THE HUMAN PROSTATEJournal of Urology, VOL. 166, NO. 6, (2484-2490), Online publication date: 1-Dec-2001.NAKADA S, JERDE T, BJORLING D and SABAN R (2018) IN VITRO CONTRACTILE EFFECTS OF NEUROKININ RECEPTOR BLOCKADE IN THE HUMAN URETERJournal of Urology, VOL. 166, NO. 4, (1534-1538), Online publication date: 1-Oct-2001.DANUSER H, WEISS R, ABEL D, WALTER B, SCHOLTYSIK G, METTLER D and STUDER U (2018) SYSTEMIC AND TOPICAL DRUG ADMINISTRATION IN THE PIG URETER: EFFECT OF PHOSPHODIESTERASE INHIBITORS α1, β AND β2-ADRENERGIC RECEPTOR AGONISTS AND ANTAGONISTS ON THE FREQUENCY AND AMPLITUDE OF URETERAL CONTRACTIONSJournal of Urology, VOL. 166, NO. 2, (714-720), Online publication date: 1-Aug-2001. Volume 160Issue 3 Part 1September 1998Page: 920-925 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information ARMIN J. BECKER More articles by this author CHRISTIAN G. STIEF More articles by this author MARKUS MEYER More articles by this author MICHAEL C. TRUSS More articles by this author WOLF G. FORSSMANN More articles by this author UDO JONAS More articles by this author Expand All Advertisement PDF downloadLoading ...