MMP-3andMMP-8single-nucleotide polymorphisms are related to alcohol-induced osteonecrosis of the femoral head in Chinese males

// Junyu Chen 1, 2, * , Wanlin Liu 1, 2, * , Yuju Cao 3 , Xiyang Zhang 4 , Yongchang Guo 3 , Yong Zhu 1, 2 , Jian Li 3 , Jieli Du 1, 2 , Tianbo Jin 5, 6, 7 , Guoqiang Wang 1, 2 , Jianzhong Wang 1, 2 1 Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, China 2 Department of the 2nd Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, China 3 Zhengzhou Traditional Chinese Medicine Traumatology Hospital, Zhengzhou, Henan 450016, China 4 School of Life Sciences, Northwest University, Xi’an, Shaanxi 710069, China 5 Key Laboratory of Molecular Mechanism and Intervention Research for Plateau Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi 712082, China 6 Key Laboratory of High Altitude Environment and Genes Related to Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi 712082, China 7 Key Laboratory for Basic Life Science Research of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi 712082, China * These authors have contributed equally to this work Correspondence to: Jianzhong Wang, email: wangjianzhongWJ@163.com Guoqiang Wang, email: chenjunyu900410@sina.com Keywords: MMP-3 , MMP-8 , alcohol-induced osteonecrosis of the femoral head, genetic, single-nucleotide polymorphism Received: September 08, 2016 Accepted: January 27, 2017 Published: February 21, 2017 ABSTRACT Our study investigated the association between MMP-3 and MMP-8 single-nucleotide polymorphisms (SNPs) and alcohol-induced osteonecrosis of the femoral head (ONFH) in 695 Chinese males (299 cases and 396 control subjects). The minor allele of MMP-3 rs650108 was associated with a 0.78-fold decrease in alcohol-induced ONFH risk in the allelic model (95% CI = 0.63-0.97, P = 0.026). In the genetic model adjusted for age, rs650108 was associated with decreased risk of alcohol-induced ONFH in the dominant model (OR = 0.68, 95% CI = 0.49-0.95, P = 0.022) and log-additive model (OR = 0.78, 95% CI = 0.63-0.98, P = 0.030); MMP-8 rs11225394 was associated with increased risk in the codominant model (OR = 1.72, 95% CI = 1.15-2.58, P = 0.010), dominant model (OR = 1.67, 95% CI = 1.12-2.48, P = 0.012), over-dominant model (OR = 1.73, 95% CI = 1.16-2.59, P = 0.007) and log-additive model (OR = 1.57, 95% CI= 1.07-2.32, P = 0.022); and MMP-8 rs2012390 was associated with decreased risk in the dominant model (OR = 0.72, 95% CI = 0.53-0.97, P = 0.032) and log-additive model (OR = 0.77, 95% CI = 0.60-0.98, P = 0.035). Haplotype analysis showed that the CGATATGT sequence mediated decreased alcohol-induced ONFH risk (OR = 0.75, 95% CI = 0.57-0.97, P = 0.029). Therefore, among Chinese males, MMP-3 rs650108 and MMP-8 rs2012390 decrease alcohol-induced ONFH risk and MMP-8 rs11225394 increases it. Further study is needed to validate our conclusion.