Chemical Recognition of Risky Habitats is Culturally Transmitted among Fathead Minnows, Pimephales promelas (Osteichthyes, Cyprinidae)

Fathead minnows (Pimephales promelas) have an alarm substance (AS), or 'Schreckstoff', in epidermal club cells. Mechanical damage to the skin, as caused by a predator attack, releases the AS. The area in which conspecifics detect AS may be considered dangerous or risky because of the high probability of a subsequent predator attack. We exposed fathead minnows to water from one of two habitats (an open‐water site and a vegetated‐cover site) that we mixed with either AS or a distilled water control. Upon subsequent exposure to water from these habitats alone, minnows showed an antipredator response to the water they experienced in conjunction with AS, but not to water they received in conjunction with the distilled water control. These results confirmed that minnows can be conditioned with AS to recognize chemical cues from high‐risk habitats. Naive minnows present during the fright response of conditioned minnows also exhibited antipredator behaviour, and subsequently responded when tested alone. Our results demonstrate that learned recognition of high‐risk habitats can be transmitted culturally, which may allow minnows to lower their risk of predation.