Further observations upon Entamoeba aulastomi Nöller

In Entamoeba aulastomi excystation does not occur in vitro until the glycogen and chromatoids have been absorbed. 2. The product of excystation is a quadrinucleate amoeba. 3. The amoeba escapes through a minute pore in the cyst wall. 4. No evidence of sexual phenomena has been obtained during the metacystic cycle. 5. The excysted quadrinucleate amoeba undergoes a series of nuclear and cytoplasmic divisions. These divisions do not necessarily synchronize or alternate with one another, but by them 8 uninucleate amoebulae containing daughter nuclei are ultimately produced from every cyst. Amoebae containing 6, 7 or 8 nuclei may appear in the course of the metacystic development.