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Anthers from the barley varieties ‘Arra’, ‘Dissa’ and ‘Ingrid’ were cultured in barley starch gelatinized nutrient media. The importance of osmosis in barley starch medium was studied by using sucrose or an inert carbohydrate, melibiose, as an osmoticum. It was found that in the barley starch medium sucrose was not necessary but energy and carbon could be obtained by tissue enzymatically from the starch. Together with the starch medium melibiose had revolutionary effects OB barley anther cultures. This system not only produced extremely high numbers of embryoids and green plantlets but also drastically reduced the number of albinos.
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Sorvari et al. (1987) studied this question.
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