Chromosome manipulation: a systematic approach toward understanding human chromosome structure and function.

in the context ofgenes orchromosomalregions that are too extensive to be easily handled withcurrentlyavailablevectors. Lastly,fromapracticalstandpoint,such chromosomes could provide a basis for novel nonviralgene therapy approaches (8).Twofundamentallydifferentapproacheshavebeentakentoaddress the requirementsnecessaryto manipulate eukaryoticchromosomes (Fig. 1). Oneinvolves