Key result
TMV 30kDa antisera precipitates multiple proteins in vitro but only one from infected tobacco protoplasts.
Population
Rabbits for antisera, TMV short rod RNA in vitro, TMV-infected tobacco protoplasts
Comparison
Antisera raised against synthetic peptide vs no antisera
Design
Preclinical study with in vitro translation and infected protoplast analysis
Follow-up
8 to 16 hours post infection
Authors
Loading...
Antisera from synthetic TMV peptide identify viral proteins in vitro; leaves open in planta validation and any translational applications.
Antisera raised against a synthetic peptide successfully identified the 30kDa protein product of the TMV 30kDa open reading frame in infected tobacco protoplasts.
Kiberstis et al. (1983) studied Tobacco Mosaic Virus (TMV) infection. Antisera raised against a synthetic peptide of the TMV 30kDa ORF was evaluated on Precipitation of proteins from in vitro translation products and TMV-infected tobacco protoplasts. Antisera raised against a synthetic peptide of the TMV 30kDa ORF precipitated 4 major proteins in vitro but only one 30.5kDa protein from TMV-infected tobacco protoplasts.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: