Mycoplasmalike organisms (MLOs) in six species of ash (Fraxinus) and lilac (Syringa) at 13 locations from southern Quebec and Massachusetts to Zion National Park, Utah, were detected by the DAPI (4'-6-diamidino-2-phenylindole.2HCl) fluorescence test. Relatedness of these MLOs to one another was established through dot hybridization of DNA samples from diseased plants with four ash yellows (AshY)-specific DNA probes and through immunofluorescence microscopy with an AshY-specific monoclonal antibody. In a search for possible alternative plant hosts of the AshY agent, the DAPI test was utilized to detect MLOs in IJ other species growing in the vicinity of diseased ash in central New York State and in two species in Zion National Park [...]
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Helen Griffiths (1994) studied this question.