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July 20, 2020HypertensionOpen Access

Ca 2+ -Dependent NOX5 (NADPH Oxidase 5) Exaggerates Cardiac Hypertrophy Through Reactive Oxygen Species Production

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Key result

NOX5 expression increases ROS production to exaggerate cardiac hypertrophy and contractile dysfunction during pressure overload.

Why the study?

The function of NOX5 in cardiac hypertrophy and its role in oxidative stress-mediated cardiac remodeling and dysfunction were unknown.

Does NOX5 overexpression exacerbate cardiac hypertrophy and contractile dysfunction in preclinical models?

Population

Cardiac tissue from patients with cardiomyopathy and heart failure, rat cardiomyocytes, and NOX5 transgenic mice

Comparison

NOX5 overexpression with Ang II or pressure overload versus controls without NOX5 overexpression

Design

Preclinical experimental study using human tissue, cell culture, and transgenic mouse models

Authors

GZGuojun ZhaoCZChang‐Ling ZhaoSOShan Ouyang

Discussion

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Overview

NOX5 may drive oxidative hypertrophy in preclinical overload; leaves open targeted inhibition in human hypertensive heart disease.

Structured PICO

Does NOX5 overexpression exacerbate cardiac hypertrophy and contractile dysfunction in preclinical models?

P
Population
Cardiac tissue from patients undergoing heart transplant for cardiomyopathy and heart failure, rat cardiomyocytes, and mice expressing human NOX5 in a cardiomyocyte-specific manner
I
Intervention
NOX5 overexpression (via adenoviral vector in rat cardiomyocytes or transgenesis in mice) combined with Angiotensin II stimulation or transverse aorta coarctation; treatment with N-acetylcysteine or diltiazem
C
Comparator
Control models without NOX5 overexpression
O
Outcome
Cardiac hypertrophy, reactive oxygen species levels, and contractile dysfunctionsurrogate

Ca2+-regulated NOX5 is an important isoform involved in oxidative stress- and MAPK-mediated cardiac hypertrophy and contractile dysfunction.

Cite This Study

Zhao et al. (2020) studied Cardiac hypertrophy. NOX5 expression vs. Control was evaluated on Cardiac hypertrophy and contractile dysfunction. Ca2+-regulated NOX5 expression increased reactive oxygen species production and exaggerated cardiac hypertrophy and contractile dysfunction in response to pressure overload and Angiotensin II.

synapsesocial.com/papers/6aae288058040f8ea87039cchttps://doi.org/10.1161/hypertensionaha.120.15558
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Biochemistry, Physiology, and Pathophysiology of NADPH Oxidases in the Cardiovascular System2012 · 763 citations
  2. 2Oxygen Free Radical Release in Human Failing Myocardium Is Associated With Increased Activity of Rac1-GTPase and Represents a Target for Statin Treatment2003 · 422 citations
  3. 3Nephropathy and Elevated BP in Mice with Podocyte-Specific NADPH Oxidase 5 Expression2013 · 127 citations
  4. 4Redox signaling, Nox5 and vascular remodeling in hypertension2015 · 93 citations
  5. 5NADPH Oxidase 5 Is a Pro‐Contractile Nox Isoform and a Point of Cross‐Talk for Calcium and Redox Signaling‐Implications in Vascular Function2018 · 70 citations