Physiochemical, circular dichroism‐induced helical conformation and optical property of chitosan azo‐based amino methanesulfonate complex

We have demonstrated the efficient procedure for the preparation of chitosan azo‐based amino methanesulfonate complex in isopropyl alcohol under mild condition. The ionic complexation between chitosan and {4‐[(4‐nitrophenyl)azo]phenyl}amino methanesulfonate is confirmed by ultraviolet (UV) and Fourier transform infrared spectroscopy. The circular dichroism spectra of chitosan complex showed positive at λ = 298 nm band with 3.88 mdeg having UV absorbance maximum up to a narrow band at λ= 290 nm in dimethyl sulfoxide, indicating that the polymer adopted helical (right handed) secondary structure. Physical properties, thermal stability, and surface morphology were analyzed by X‐ray diffraction, thermogravimetric analysis, differential scanning calorimetry and scanning electron microscopy. Optical properties of chitosan complex are evaluated by photoluminescence spectroscopy that showed blue shift (λem) peak at 400 nm at excitation wavelength 325 nm. Overall, it may open new perspectives for the use of chitosan azo‐based biohybrid in biomedical applications.