In order to test our hypothesis that the genuine pathogen of the pine wilt disease mediated by Bursaphelenchus xylophilus is some species of phenylacetic acidproducing bacteria accompanying nematodes, we inoculated aseptic pine seedlings with aseptic nematodes. It was found that 1) phenylacetic acid was not the product of the nematode but that of the accompanying bacteria, and 2) the aseptic pine seedling inoculated under aseptic conditions with the aseptic nematode did not wilt. This finding confirms our hypothesis.
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Kawazu et al. (1997) studied this question.
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