Binding of 3H‐Methyltrienolone (3H‐R1881) to androgen receptors in human gingiva

Binding of 3H‐Methyltrienolone (3H‐R1881) to cytoplasmic androgen receptor protein and its translocation into nuclei in human gingiva was demonstrated. 3H‐R1881 binds to androgen receptors with high affinity (Kd= 1.7−1.9 × 10−9 M) and low capacity (10–16 fmol/mg protein). The binding was specific for DHT binding sites and was destroyed by proteolytic enzymes (pronase and trypsin). These results suggest that 3H‐R1881 can be used as a ligand in combination with nonradioactive DHT to study androgen receptors in the gingiva.