Effects of amantadine on uptake and release of dopamine by a particulate fraction of rat basal ganglia

Amantadine, in vitro, produced dose-dependent blockade of dopamine uptake into a synaptosome-rich particulate fraction of an homogenate of the basal ganglia of rats. A concentration of 3·6 times 10−6M was required to inhibit uptake by 50%. Diethazine was less potent in blocking dopamine uptake, the equiactive concentration being 8·0 times 10−6M. Amantadine and (+)-amphetamine in concentrations in excess of 10−5M caused the release of small quantities of added dopamine from the particulate fraction.