Carboxyl proteinase from the wood deteriorating basidiomycete Pycnoporus coccineus: Substrate specificity with oxidized insulin peptide B1-B16 and B15-B24,angiotensin and proangiotensin.

The specificity of highly purified carboxyl proteinase from Pycnoporus coccineus (formerly designated Trametes sanguined) was investigated with oligopeptides at pH 2.7.Hydrolysis of oxidized insulin peptide Bl ~B16 was observed at two peptide bonds (Hislo-Leun and Ala14-Leu15) during 3-hr incubation.The enzyme did not hydrolyze oxidized insulin peptide B15~B24.