Dragging it out: The first direct quantitative analysis of multiple miRNAs (DQAMmiR) uses miRNAs directly, without chemical or enzymatic modification, and accurately determines concentrations of multiple miRNAs without the need for calibration curves. This method involves a capillary-electrophoresis-based hybridization assay with a combination of two separation-enhancement approaches: drag tags and single strand DNA binding protein (SSB).
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Wegman et al. (2011) studied this question.
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