The objective of this research was to saccharify cassava flour by acid-acid and acid-enzyme hydrolysis and further conversion of the resulting sugar into ethanol by fermenting with the immobilized (in Ca-alginate) cells of Saccharomyces cerevisiae . The saccharification resulted in higher total sugar recovery by acid-enzyme hydrolysis (72.88 %) than by enzyme-enzyme hydrolysis (58.1 %). Further study on ethanol production was carried out using the hydrolysate obtained from acid-enzyme hydrolysis. The growth of the yeast started in the log phage and maximum ethanol (189 ± 3.1 g ethanol/kg flour) production was achieved with 94.74 ± 2.187 % sugar conversion during the stationary phase.
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Behera et al. (2014) studied this question.
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