Cloning and expression of the antitumor glycoprotein gene of Streptococcus pyogenes Su in Escherichia coli.

The structural gene for the streptococcal acid glycoprotein (SAGP), an antitumor glycoprotein produced by Streptococcuspyogenes Su, was cloned in Escherichia coli, and the nucleotide sequence was determined.The deduced amino acid sequence of SAGPwas composed of410 amino acids, and was highly homologous with colicin El, a bacterial antimicrobial protein.The SAGPgene was subcloned into an expression plasmid and was expressed in E. coli.The gene product was