Key result
SV40 large T activation at 33°C abolishes probenecid-sensitive cyclic nucleotide extrusion polarity in rabbit collecting ducts.
Why the study?
The physiological role and polarity of ANP-stimulated cGMP egression in renal principal cells and the effect of SV40 large T antigen on this process were unclear.
Population
Rabbit collecting duct cell line transformed with temperature-sensitive SV40
Comparison
ANP-stimulated cGMP vs isoproterenol-stimulated cAMP egression at different temperatures and with inhibitors
Design
In vitro cell line study with temperature modulation and pharmacological interventions
Authors
Loading...
Provides novel in vitro model for ANP-cGMP egression in principal cells; leaves open translation to human renal physiology.
The study demonstrates a physiological role for luminal cGMP in the rabbit collecting duct and a specific effect of SV40 large T on the polarity of probenecid-sensitive carrier extrusion.
Millul et al. (1996) studied this question. Activation of SV40 large T at 33 degrees C vs. Inactivated large T at 39.5 degrees C was evaluated on Polarity of probenecid-sensitive cyclic nucleotide extrusion. Activation of SV40 large T at 33 degrees C abolished the polarity of probenecid-sensitive cyclic nucleotide extrusion in a rabbit collecting duct cell line.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: