Key result
Small interfering RNA targeting the A151R and VP72 genes reduced African swine fever virus replication by up to 4 log10 copies and RNA transcripts by up to 3 log10 copies in vitro.
Why the study?
There is no vaccine or treatment available to control African swine fever virus, and its replication control strategies are needed.
Does siRNA targeting the A151R and VP72 genes reduce African swine fever virus replication in vitro?
Does siRNA targeting the A151R and VP72 genes reduce African swine fever virus replication in vitro?
siRNA targeting A151R and VP72 genes effectively inhibits African swine fever virus replication in vitro, providing a potential strategy against a virus that replicates exclusively in the cell cytoplasm.
Hypothesis-generating for siRNA-based ASFV therapy; leaves open in vivo efficacy and precludes clinical use.
BACKGROUND: African swine fever virus (ASFV) is the unique member of the Asfarviridae family and Asfivirus genus. It is an enveloped double-stranded DNA arbovirus that replicates in the cell cytoplasm, similar to poxviruses. There is no vaccine and no treatment available to control this virus. METHODS: We describe the use of small interfering RNA (siRNA) targeting the A151R and VP72 (B646L) genes to control the ASFV replication in vitro. RESULTS: Results suggest that siRNA targeting the A151R and VP72 genes can reduce both the virus replication and its levels of messenger RNA transcripts. The reduction was up to 4 log(10) copies on the virus titre and up to 3 log(10) copies on virus RNA transcripts levels. The combination of multiple siRNA did not improve the antiviral effect significantly, compared with use of individual siRNAs. CONCLUSIONS: The function of the A151R gene product in the virus replication cycle is yet unclear, but is essential. We also demonstrate that it is possible to inhibit, using small interfering RNA, a virus that replicates exclusively in the cell cytoplasm in specific viral factories.
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Keita et al. (2010) studied African swine fever virus (ASFV) infection. small interfering RNA (siRNA) targeting the A151R and VP72 genes was evaluated on ASFV replication and messenger RNA transcripts levels. Small interfering RNA targeting the A151R and VP72 genes reduced African swine fever virus replication by up to 4 log10 copies and RNA transcripts by up to 3 log10 copies in vitro.
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