Dynamic lipid heterogeneity and receptor events

Receptor occupation by specific ligands induces changes in the dynamic domain organization of surrounding lipids. Such changes were observed by measuring changes in the fluorescence parameters of fluorescent-labelled lipids incorporated into plasma membranes of intact cells, membrane vesicles or lipoprotein particles in response to specific binding of a broad range of biologically active agents, including drugs, prostaglandins, neuropeptides, antibodies and viruses. The high sensitivity of the fluorescence response allowed us to register changes in lipid heterogeneity induced in a multitude of discrete targets by transient weak binding of a single rapidly translocating molecule. To explain these observations a non-equilibrium model of ligand-receptor interaction based on low relaxation phenomena in heterogeneous lipid matrixes is proposed.