Antimicrobial Effects of Propolis Extracts on Escherichia coli and Staphylococcus aureus Strains Resistant to Various Antibiotics and Some Microorganisms

Propolis is collected by worker honey bees from the trees. The bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. Propolis has also been used by humans since early times for various purposes and, especially, as a medicine because of its antimicrobial properties. In this study, antimicrobial effects of propolis samples on three Escherichia coli and two Staphylococcus aureus strains that are multiresistant to antibiotics and on Salmonella typhimurium, Bruceila abortus, Proteus mirabilis, Streptococcus pyogenes, Bacillus cereus, Candida tropicalis, and Aspergillus niger were studied. Propolis extracts were prepared with acetone and dimethyl sulfoxide (DMSO). At concentrations of 10 and 25 ml, propolis extracts affected all of the microorganisms in acetone and DMSO in concentrations of 10.0, 50.0, and 100.0 ppm. The results detected in this in vitro study provided evidence that propolis may be used as an antimicrobial agent against many microorganisms.