EXAFS investigation on the iron(III) binding sites of hen phosvitin

phosvitin from chicken eggs in water solution with an iron/protein molar ratio of 10/1 at pH 7.2. The main result is that the atoms are bound to the protein in an octahedral environment and that the main binding sites are provided by the oxygen atoms serine-bound phosphate groups at 1.93 (2) A from the metal. The average number of bound phosphate groups is 4.4 (9) per atom, hexacoordination being achieved upon binding of other possible donors from the protein or solvent water molecules. evidence has been found of short Fe-Fe interactions, so that, on the basis of our data, it can be stated that iron is not essentially in a polynuclear structure with Fe-0-Fe bridges.