SUMMARY: The methods described allowed successful cultivation of 60 % of single cell isolations in the case of an aerobic, transiently chain-forming and polymorphous organism; 40 % in the case of an organism of related morphology and similar ready viability, but presenting technical difficulties because of the lipophilic nature of the surface membranes; and approximately 2 % in the case of a micro-aerophilic or anaerobic organism of restricted viability.
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Erikson et al. (1954) studied this question.
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