Effect of Grape Replant on The Soil Microbial Community Structure and Diversity

ABSTRACTAt present, replant obstacle has become an important problem in grape production, and it has seriously restricted the development of the grape industry. This study compared the soil from a replant vineyard and a newly planted vineyard where vine had been grown for 30 years and 3 years respectively. A pot experiment was conducted to investigate the changes of plant growth when grape was grown on root zone soil, sterilized root zone soil and fallow soil from the corresponding vineyards. The variation of the microbial population of the rhizosphere soil, non-rhizosphere soil and fallow soil from different vineyards was studied based on PCR-DGGE approach. The amplified bands of the dominant population were sequenced. The results showed that the plants growth was suppressed by vine replanting, and the plants grew significantly stronger after replant soil sterilization. The bacterial and fungal diversity increased as the period of the grape planting extended. The diversity of the microbial population in ...