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In the Tajima strain, there are three substrains called Nakadoi, Kumanami and Kinosaki substrains.Out of those, we used two main substrains, Nakadoi and Kinosaki.Blood samples were collected from representative 252 dams from Genealogical Relationship between Pedigree and Microsatellite Information and Analysis of Genetic Structure of a Highly Inbred Japanese Black Cattle Strain
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Sasazaki et al. (2004) studied this question.
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