Bioanalytical Method Optimization for the Therapeutic Drug Monitoring of Vancomycin

Chemometric optimization and validation of a method based on High Performance Liquid Chromatography (HPLC) using core – shell particles for the determination of Vancomycin (VMC) in human plasma is reported. The combination of the efficiency of the core-shell particles and the benefits of the design of experiments allowed the successful determination of VCM, even in presence of several interferents. Selectivity, linearity, accuracy and precision were accomplished according to the European Medicines Agency (EMA) guideline, within the concentration range of 1.00 – 60.0 μg/mL of VCM. It is noteworthy that this method requires small amount of sample and solvents, and the sample treatment is simple and no time-consuming. Thus, this method becomes a simple and high-throughput alternative to therapeutic drug monitoring in treated patients, as well as an analytical procedure that conforms to the principles of the green chemistry.