Key result
Measles virus RNA is absent in otosclerosis samples, failing to support a causal link.
Why the study?
The presence of measles virus genetic material in the stapes of patients with otosclerosis was uncertain and needed molecular evaluation.
Is measles virus genetic material present in the stapes of patients with otosclerosis?
Cross-Sectional (n=93)
No
Is measles virus genetic material present in the stapes of patients with otosclerosis?
This study found no evidence of measles virus genetic material in the stapes of patients with otosclerosis, challenging the theory that measles virus is a causal factor.
Answers are grounded in this paper and cite their sources.
Questions measles causality in otosclerosis; leaves open alternative etiologies for prospective study.
Objective To determine molecularly the presence of measles virus genetic material in the stapes of patients with otosclerosis. Study Design A cross-sectional study. Setting A tertiary referral hospital. Subjects and Methods Genetic material was extracted from the stapes of patients with otosclerosis (n = 93) during the period from March 2011 to April 2012. The presence of viral measles sequences was evaluated by the real-time reverse transcriptase polymerase chain reaction (RT-PCR). The expression of the CD46 gene was determined. Results Ninety-three patients were included in the study. No sample was positive for any of 3 measles virus genes (H, N, and F). Measles virus RNA was not detected in any sample by real-time RT-PCR. CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90). Conclusion This study does not support the theory of measles virus as the cause of otosclerosis. It is necessary to do more research about other causal theories to clarify its etiology and prevention.
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Flores-García et al. (2017) conducted a cross-sectional in Otosclerosis (n=93). Measles virus genetic material was evaluated on Presence of measles virus genetic material (H, N, and F genes) by real-time RT-PCR. Measles virus RNA was not detected in any of the 93 stapes samples from patients with otosclerosis, failing to support the theory of measles virus as the cause of the disease.
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