Key result
Jinchai capsule reduces HA and PA mRNA expression and lowers intracellular pH in influenza A-infected cells.
Why the study?
The antiviral effects of Jinchai capsule against influenza virus and its mechanisms were evaluated in vitro.
Does Jinchai capsule inhibit influenza virus A infection in MDCK cells?
Does Jinchai capsule inhibit influenza virus A infection in MDCK cells?
Jinchai capsule demonstrates in vitro antiviral activity against influenza A virus by inhibiting viral adsorption, fusion, transcription, and replication.
In vitro antiviral activity of Jinchai capsule is hypothesis-generating; leaves open any clinical role in influenza.
OBJECTIVE: To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine. METHODS: Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin (HA) protein, intracellular pH, and influenza virus protein acid (PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction. RESULTS: Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells. Jinchai also significantly decreased intracellular pH in infected cells. CONCLUSIONS: Jinchai had strong anti-influenza activity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replication of the virus.
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Zhong et al. (2013) studied Influenza virus A (subtype H1N1) infection. Jinchai capsule was evaluated on Expression of HA and PA polymerase subunit mRNA, and intracellular pH. Jinchai capsule significantly reduced the expression of HA and PA polymerase subunit mRNA and decreased intracellular pH in MDCK cells infected with influenza virus A.
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