Key result
IL13 increases periostin and STAT6 in cultured osteoarthritis synoviocytes, which dexamethasone and leflunomide inhibit.
Why the study?
The reaction cascade from IL13 to periostin in osteoarthritis synovial fluid remains unidentified, motivating investigation of periostin secretion downstream of IL13.
IL13 up-regulates periostin in osteoarthritis synoviocytes via STAT6, an effect that can be inhibited by dexamethasone and leflunomide.
IL-13/STAT6 signaling may promote periostin in OA synoviocytes; hypothesis-generating and should not yet change practice.
BACKGROUND: Our previous research provided evidence of periostin increase in parallel with interleukin-13 (IL13) increase in the synovial fluid of patients with osteoarthritis (OA). The reaction cascade from IL13 to periostin, however, remains unidentified. We, therefore, tested the hypothesis that periostin secretion is affected downstream of IL13. MATERIALS AND METHODS: OA synoviocytes were cultured under different concentrations of IL13. Periostin content in culture supernatants and the level of signal transducer and activator of transcription 6 (STAT6) in the cultured cells were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the influence of dexamethasone and leflunomide on periostin production in relation to the effect of IL13 on the cells was also examined. RESULTS: Periostin content in culture supernatants and the level of STAT6 in cultured cells were significantly increased by IL13. The increase of periostin was significantly inhibited by dexamethasone and leflunomide. CONCLUSION: Periostin may be up-regulated in OA synoviocytes via STAT6 downstream of IL13.
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Moue et al. (2017) studied Osteoarthritis. IL13 was evaluated on Periostin content in culture supernatants and STAT6 levels in cultured cells. IL13 significantly increased periostin content and STAT6 levels in cultured osteoarthritis synoviocytes, an effect that was significantly inhibited by dexamethasone and leflunomide.
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