Development and Validation of a Solid-Phase Extraction Gas Chromatography-Mass Spectrometry Method for the Simultaneous Quantification of Opioid Drugs in Human Whole Blood and Plasma

A rapid, selective, sensitive, and specific method is presented to simultaneously quantify morphine, 6-monoacetylmorphine (6-MAM), codeine, heroin, fentanyl, and methadone in human whole blood and plasma.The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction (SPE) and quantification by GC/MS with electron impact ionization using helium as carrier gas.Quantification was performed based on nalorphine as internal standard (IS).The specificity, linearity, intra-and inter-assay precision and accuracy, and extraction recovery were fully evaluated.The limits of detection (LODs) were 0.40 -7.63 ng/mL for whole blood and 0.80 -32.00 ng/mL for plasma.The method is fast, simple, and accurate, with the sensitivity and specificity required in forensic and clinical toxicology.