A Streptomyces Sp strain producing appreciable levels of cholesterol oxidase was selected. In the culture medium, cholesterol oxidase activity can reach 13,000 UI/l. Cholesterol oxidase was purified by means of centrifugation, tangential ultrafiltration, ion-exchange chromatography and gel filtration with a yield of 42% to a specific activity of 54 UI/mg. Its molecular weight determined by SDS-PAGE was 55,000. Its optimum activity pH is about 7.
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Lartillot et al. (1990) studied this question.
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