Quantification of Callose Deposition in Plant Leaves

Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is in higher plants. Callose is involved in multiple aspects of plant growth and . It is synthetized in plants at the cell plate during cytokinesis, in several stages pollen development and is deposited at plasmodesmata to regulate the cell-to-cell of molecules. Moreover, it is produced in response to multiple biotic and abiotic (Chen and Kim, 2009). Callose is considered to act as a physical barrier by the plant cell well to slow pathogen infection and to contribute to the plant’s immunity. Thus the callose staining method is useful to quantify activity of plant immunity. addition, this staining can be used to visualize structures in plant tissue, where the callose be implied whether during the development of plants or response against pathogen . This method is based on the use of methyl blue which reacts with (13)--glucans to a brilliant yellow fluorescence in UV light. Moreover, calcofluor stains chitin present in cell membranes and also binds to cellulose at locations where the cuticle is damaged.