Key result
Lower F5 and higher Protein S DNA methylation levels are linked to thrombophilia.
Why the study?
The relationship between leukocyte DNA methylation at targeted sites and thrombophilia was not well understood.
Is targeted DNA methylation in peripheral leukocytes associated with thrombophilia?
Case-Control (n=16)
No
Is targeted DNA methylation in peripheral leukocytes associated with thrombophilia?
Targeted DNA methylation in Protein S.44.CpG.29-33 and F5.24.CpG.10 islands may serve as biomarkers for elevated risk of thrombophilia.
Methylation differences at these sites should not yet inform thrombophilia care; leaves open their value as biomarkers or mechanistic clues.
Objective The aim of this study is to investigate the relationship of leukocytes DNA methylation in targeted sites and thrombophilia. Methods Eight thrombophilia patients and their kin-related individuals as the healthy control. Targeted DNA methylation from peripheral leukocytes were examined with MassArray. Multivariate correlation analysis was used to estimate targeted gene methylation as an independent risk factor of thrombophilia. Receiver operating characteristic curve analysis was used to calculate the accuracy of biomarkers in the prediction of thrombophilia. Results The age of thrombophilia group was higher than control group (P < 0.001). F5.24.CpG.10 and Protein S.44.CpG.29–33 methylation were significantly associated with thrombophilia negatively and positively (r = -0.7289, P < 0.01 and r = 0.5667, P < 0.05). F5.24.CpG.10 methylation was higher in control group (P < 0.01), but Protein S.44.CpG.29–33 methylation increased in thrombophilia group (P < 0.05). The areas under curve of ROC were 0.9297 and 0.8437, respectively. Conclusion Target DNA methylation in Protein S.44.CpG.29–33 island is associated with an elevated risk of thrombophilia.
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Xiang et al. (2024) conducted a case-control in Thrombophilia (n=16). Targeted DNA methylation (F5.24.CpG.10 and Protein S.44.CpG.29-33) vs. Healthy kin-related individuals was evaluated on Correlation of F5.24.CpG.10 methylation with thrombophilia (r = -0.7289, p=<0.01). Targeted DNA methylation levels at F5.24.CpG.10 were significantly lower and at Protein S.44.CpG.29-33 were significantly higher in patients with thrombophilia compared to healthy controls.
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