Growth and Regeneration of Schistosotna mansoni in vitro.

Preliminary investigations on the growth of Schistosoma mansoni in vitro are reported. In a nutrient medium composed of bovine amniotic fluid, beef embryo extract, and horse serum, schistosomulae generally showed evidence of growth. The duration of survival was directly related to initial size. Worms that initially approximated 1.0 mm or greater in length were found to survive many weeks in vitro; the survival period of smaller schistosomulae was of much shorter duration.