Key result
New in vitro methods determine antiviral toxicity and inhibitory concentrations against herpes simplex and adenovirus.
Why the study?
The increasing frequency of life-threatening viral diseases in immunodepressed hosts requires comparative in vitro data on antiviral drug activity for clinical decision-making.
This study presents an in vitro methodology for determining tissue-viral susceptibility ratios of antiviral drugs to potentially guide clinical treatment choices.
Supports lab-guided antiviral selection in immunodepressed patients; leaves open clinical translation of in vitro findings.
The availability of drugs such as cytosine arabinoside, iododeoxyuridine, and fluorodeoxyuridine has given the clinician choices with regard to treatment of life-threatening viral diseases that are occurring with increasing frequency in immunodepressed hosts. This choice of therapy places increased responsibility on the clinical virology laboratory to make available comparative in vitro data regarding the activity of antiviral drugs against viruses isolated from patients. Presented in this paper are methods developed for determining the minimal toxicity concentrations of antiviral drugs against the tissue culture cell lines routinely used in isolating viruses. Also presented are methods for determining the minimal inhibitory concentration of antiviral drugs against two strains of herpes simplex virus and one strain of adenovirus. These two values give a tissue–viral susceptibility ratio for each drug. As new drugs become available, these methods of testing in vitro may well assume clinical importance.
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Walker et al. (1971) studied Viral diseases. Antiviral drugs (cytosine arabinoside, iododeoxyuridine, fluorodeoxyuridine) was evaluated on Minimal toxicity concentrations and minimal inhibitory concentrations. In vitro methods were developed to determine the minimal toxicity and inhibitory concentrations of antiviral drugs against herpes simplex and adenovirus to calculate tissue-viral susceptibility ratios.
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