Key result
Affinity chromatography isolates Aleutian disease virus, revealing two distinct virus-antibody complexes.
Hypothesis-generating for isolating persistent viruses; leaves open clinical relevance of complex dissociation patterns.
Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.
No takes yet. Share an insight, caveat, or question.
Kenyon et al. (1973) studied Aleutian disease virus of mink. Affinity chromatography was evaluated on Isolation of Aleutian disease virus. Affinity chromatography successfully isolated the Aleutian disease virus of mink, revealing two different types of virus-antibody complexes based on their dissociation requirements.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: