Eggs of the American lobster, Homarus americanus, were examined for estrogenic materials. Analysis by the Astwood 6-hour assay indicated 0.26 to 0.32 μg of estrogenic activity per kilogram of eggs. Analysis by countercurrent distribution, paper chromatography, and bio-assay strongly suggests that the biologically active material is estradiol-17β.
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Robert D. Lisk (1961) studied this question.
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