Preharvest kernel infection by Aspergillus flavus and subsequent aflatoxin contamination of maize (Zea mays) grain is a chronic economic problem in the southern part of the United States. Studies were conducted to determine the time of inoculation and harvest date most effective for identifying maize genotypes resistant to kernel infection and aflatoxin contamination by A. flavus. Inoculation of ears with A. flavus 6 days after midsilk resulted in as many or more infected kernels than inoculation of ears 12 and 18 days after midsilk. Multiple inoculations did not increase incidence of kernel infection or aflatoxin contamination
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Gene E. Scott (1994) studied this question.