Glomerular expression pattern of long non-coding RNAs in the type 2 diabetes mellitus BTBR mouse model

Figures

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  Figure 1. Expression of lncRNAs in BTBR ob/ob mice compared to age-matched WT mice. (A) Expression pattern of lncRNAs in 8, 16, and 24 weeks old BTBR ob/ob mice vs. BTBR WT mice categorized in sense and antisense lncRNAs, as well as lincRNAs with or without neighboring mRNA within a distance of 50 kb up- or downstream. The yellow part of each bar represents the number of upregulated transcripts and the blue part represents the number of downregulated transcripts. (B) The Venn diagram shows the total number of differentially expressed lncRNAs per age group.

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  Figure 2. Percentage of differentially expressed mRNAs and lncRNAs per chromosome.

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  Figure 3. Validation of glomerular gene expression by qPCR in the course of time in BTBR WT and BTBR ob/ob mice and the result of the microarray for 24 weeks old mice (for selected genes). The qPCR analysis was performed for the following ages: four, eight, twelve, 16, 20, and 24 weeks (4 w, 8 w, 12 w, 16 w, 20 w, 24 w), also presented the result of the microarray analysis for 24 weeks old mice (24w). For qPCR and microarray analyses, we used RNA extracted from sieved glomeruli from 3–6 animals (details see below) as independent samples. (A) Itpr1 and its sense lncRNAs NONMMUT058318, NONMMUT058319, and NONMMUT058320. (B) Rere and its sense lncRNAs NONMMUT050705 and NONMMUT050715. (C) Nphs1 and its antisense lncRNA Nphs1as. (D) Acsm1 and its antisense lncRNAs uc009jll.1 and Gm45792. (E) Wt1 and its neighboring lncRNA NONMMUT038946. (F) Casr and its neighboring lncRNA NONMMUT026469. Black bars represent the fold change of the protein-coding gene for age-matched murine genotypes. Red, blue or yellow bars correspond to the associated sense, antisense, or neighboring lncRNA for age-matched murine genotypes. Error bars indicate the standard deviation of the fold change. For qPCR experiments: The…

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  Figure 4. Localization of glomerular expressed transcripts using RNA from murine mesangial cells (mMC), murine glomerular endothelial cells (mGlend), and murine podocytes (mPC). (A) Itpr1 and its sense lncRNAs NONMMUT058318, NONMMUT058319, and NONMMUT058320. (B) Rere and its sense lncRNAs NONMMUT050705 and NONMMUT050715. (C) Nphs1 and its antisense lncRNA Nphs1as. (D) Acsm1 and its antisense lncRNAs uc009jll.1 and Gm45792. (E) Wt1 and its neighboring lncRNA NONMMUT038946. (F) Casr and its neighboring lncRNA NONMMUT026469. Values represent the ratio of the CT of the transcript vs. CT of cyclophilin B. A low ratio (<1.0) indicates a high expression and is highlighted in yellow, while a high ratio (>1.8) indicates a low expression and is highlighted in blue.—portends that no expression was detected.