Amycolasporins and Dibenzoyls from Lichen-Associated Amycolatopsis hippodromi and Their Antibacterial and Anti-inflammatory Activities

Eleven metabolites, six echinosporins ( 1 – 6 ), four dibenzoyls ( 7 – 10 ), and an aromatic compound ( 11 ), were isolated from the fermentation broth of lichen-associated Amycolatopsis hippodromi . The structures of the new compounds ( 1 – 5, 8 – 11 ) were elucidated by comprehensive spectroscopic analysis including data from experimental and calculated ECD spectra. Amycolasporins A–C ( 1–3 ) demonstrated antibacterial activities against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli with MIC values of 25 or 100 μg/mL. Amycolasporin C ( 3 ) and the known dibenzoyl ( 7 ) attenuated the production of NO due to the suppression of the expression of nitric oxide synthase (iNOS) in LPS-induced RAW 264.7 cells in a dose-dependent manner.