Vacuum-Ultraviolet Circular Dichroism Spectrophotometer Using Synchrotron Radiation: Optical System and On-line Performance

With the aim of measuring the circular dichroism (CD) spectra of biomaterials in aqueous solutions in the vacuum ultraviolet region between 310 and 140 nm, an optical system withstanding high vacuum was incorporated into the beam line 15 of Hiroshima Synchrotron Radiation Center (HiSOR). The performance of the constructed system was confirmed to be satisfactory in the high-vacuum on-line experiments by monitoring the CD spectra of ammonium d-camphor-10-sulfonate, d- and l-alanines in water.