In vitro–grown leaves of Malus × domestica Borkh. cv. Royal Gala were either crush-wounded with forceps, cut, or left whole, and then inoculated with A. tumefaciens strain EHA105 (p35SGUS_INT), with and without vacuum infiltration. Transformation was quantified 13 days after inoculation by determining the rate of β-glucuronidase (GUS) activity. Leaf wounding by crushing with nontraumatic forceps significantly increased transformation when compared with cutting leaves. Vacuum infiltration of inoculum had no effect on transformation.
No takes yet. Share an insight, caveat, or question.
Norelli et al. (1996) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: