An extended-cavity Cr:forsterite laser is integrated with a photonic-crystal fiber soliton frequency shifter and a periodically poled lithium niobate spectrum compressor for simultaneous harmonic-generation and coherent Raman brain imaging. Adapting the laser beam focusing geometry to the tissue morphology is shown to enable complementarity enhancement in tissue imaging by second- and third-harmonic generation, as well as coherent Raman scattering, facilitating quantitative image analysis.
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Amitonova et al. (2011) studied this question.
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