Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231

Enterococcus faecium CCM4231, isolated from the rumen content of a calf, produced an antimicrobial agent active against Gram‐positive and Gram‐negative indicator organisms. After 100‐fold concentration by ultrafiltration, the diameters of zones of inhibition ranged from 2 to 10 mm. The agent was sensitive to pronase and trypsin and resistant to heating at 60°C for 30 min.