Detection of heavy metals from Harpadon nehereus, Channa punctata and Pampus chinensis dried fishes and toxicity study of Channa punctata on embryonic zebrafish

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Globally, heavy metal pollution is one of the major serious environmental issues. The present investigations were carried out to determine the heavy metals concentrations from three dried fish along with the toxicity effects of Channa punctata dried fish on embryonic development of zebrafish. Methods: The concentrations of heavy metals, Nickel (Ni), Chromium (Cr), Cadmium (Cd), Arsenic (As), and Lead (Pb) were detected from Harpadon nehereus, C. punctata and Pampus chinensis dried fish’s samples by Atomic Absorption Spectrophotometry (AAS) method. Results: The highest concentrations of As and Pb were 3.65±0.08 and 3.29±0.07 ppm, found in C. punctata followed by 3.11±0.05 and 3.26±0.07 ppm, in H. nehereus, respectively. Meanwhile, no significant levels of Ni, Cr and Cd were found in all the three tested dried fishes, compared with standard references. The treatments of 50µg/ml and 100µg/ml C. punctata dried fish solution on zebrafish embryos showed significant toxicity and abnormalities of unequal blastomeric curve, yolk sac edema, pericardial edema, spinal curvature and tail deformation at 24, 33, 48, 72 and 96 hour post fertilization (hpf). Conclusions: The present investigation revealed that the tested dried fishes contain heavy metals and C. punctata is highly toxic for zebrafish embryos.