Key result
Long-term individual shear rate therapy linked to increased plasma nitrite release in PAD patients.
Why the study?
Individual shear rate therapy (ISRT) is a novel non-invasive treatment for PAD patients, but its impact on nitric oxide levels and molecular markers was unclear.
Does individual shear rate therapy (ISRT) improve plasma nitrite release and alter NOS2 expression in healthy subjects and patients with peripheral artery disease?
Does individual shear rate therapy (ISRT) improve plasma nitrite release and alter NOS2 expression in healthy subjects and patients with peripheral artery disease?
Long-term individual shear rate therapy increases plasma nitrite release in patients with peripheral artery disease, suggesting improved endothelial function comparable to physical exercise.
Long-term ISRT raises plasma nitrite in PAD patients; hypothesis-generating for NO mechanisms, larger trials needed before practice change.
BACKGROUND: Individual shear rate therapy (ISRT) has been designed as a novel non-invasive treatment option for peripheral artery disease (PAD) patients and has been shown to improve endothelial function and walking distance. The aim of this study was to elucidate the impact of ISRT on the level of nitric oxide in patient blood plasma and the expression of related molecular markers in peripheral blood mononuclear cells (PBMCs). Molecular diagnostic tests were performed for two ISRT trials. PATIENTS AND METHODS: In ISRT-1 26 healthy subjects underwent one session of treadmill training and one session of ISRT respectively in a cross-over design. In ISRT-2 14 PAD patients with a stable intermittent claudication underwent a 30 hours long-term treatment. Plasma nitrite release as well as the mRNA expression of NOS2 and key regulators of the kallikrein-kinin system were measured in PBMCs at different time points. RESULTS: Short-term ISRT revealed significantly decreased NOS2 expression in PBMCs of healthy volunteers and PAD patients. Long-term ISRT, in turn, demonstrated a significant plasma nitrite increase in PAD patients. CONCLUSIONS: We verified that long-term ISRT stimulates the vascular system and exerts a comparable effect to physical exercise in regards to NO release, which coincide with recent findings regarding an improvement of endothelial function. However, further studies are necessary to investigate the role for circulating leukocytes. .
No takes yet. Share an insight, caveat, or question.
Brix et al. (2016) studied Peripheral artery disease (PAD) (n=40). Individual shear rate therapy (ISRT) vs. Treadmill training (for short-term cross-over) was evaluated on Plasma nitrite release and mRNA expression of NOS2. Long-term individual shear rate therapy significantly increased plasma nitrite release in patients with peripheral artery disease.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: