Development and Mapping of Microsatellite Markers Derived from cDNA in Japanese Quail (Coturnix japonica)

The objective of this work was to develop polymorphic microsatellite markers derived from Japanese quail embryonic and cardiac cDNA libraries and to locate these markers on Kobe-NIBS Japanese quail (KNQ) linkage map constructed mainly by AFLP markers in previous study.Of the 20 positive clones, /+ clones were unique and identified as genes or ESTs by BLAST searches, and ,/ from /+ clones were possible to design the PCR primers after partial sequencing.Only six markers were polymorphic and used by linkage analysis in KNQ resource family.These markers were mapped on four linkage groups, JQG +, JQG 3, JQG +3 and JQG 00, and then these linkage groups were assigned to chromosome of Japanese quail, CJA ,, CJA +., CJA +1 and CJA ,-, respectively.These markers derived from cDNA are useful and powerful tool, and contribute to the comparative map between Japanese quail and the other species.