Study of the Volatile Compounds in Artemisia Sagebrush from Iran using HS/SPME/GC/MS

The aerial parts of Artemisia Sagebrush were collected in August 2010 from Damavand ( Tehran Province of Iran ).It is air dried in a shadow place.A headspace solid-phase micro-extraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC/MS) was used for the analysis of volatile compounds in the leaves of Artemisia Sagebrush.The dried plant was powdered and the aroma compounds of a 0.05g were isolated by HS-SPME fiber in 15 min.The chemical compounds of plant were investigated by gas chromatography mass spectrometry (GC/MS).The oven temperature of GC was programmed between 60-250°C using DB-5 column.A total of 51 constituents, representing more than 96%of the volatiles were identified.The main compounds, were isobornyl acetate (19.56%) , eucalyptol (14.81%), (+) trans-chrysanthenyl acetate (12.76%), αcubene (9.70%), borneol (9.06%), caryophyllene (7.03%), camphor (6.83%), γ-elemene (2.16%), umbellul one (1.73%) and copaene (1.37%).This herbal medicine traditionally uses as antiinfectious, anti-bacterial, gastric tonic, digestive and stomachic in Iran.Consequently, this fast and simple method can be used for the analysis of the volatile compounds emitted from Artemisia Sagebrush.